Goat Anti-IGFBP3 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

growth hormone-dependent binding protein|binding protein 53|binding protein 29|acid stable subunit of the 140 K IGF complex|IGF-binding protein 3|IBP3|BP-53|tcag7.703|insulin-like growth factor binding protein 3|IGFBP3

NP_001013416.1; NP_000589.2

EBP08005

Peptide with sequence C-RYKVDYESQSTDTQN, from the internal region of the protein sequence according to NP_001013416.1; NP_000589.2.

This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2).

C-RYKVDYESQSTDTQN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog

Human

3486

16009

24484

Antibody detection limit dilution 1:64000.

Approx 28kDa band observed in Human Breast Cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). Recommended concentration: 0.02-0.06µg/ml. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Pep-ELISA, WB