Goat Anti-Proenkephalin Antibody

$423.00

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SKU: EB08195
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
PENK|proenkephalin
Accession ID
NP_006202.1
Blocking Peptide
EBP08195
Immunogen
Peptide with sequence C-RSHHQDGSDNEE, from the internal region of the protein sequence according to NP_006202.1.
Peptide Sequence
C-RSHHQDGSDNEE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
5179
Product Grade
IHC Results
Frozen section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus. Recommended concentration: 0.3-1µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 37kDa band observed in Human Adrenal Gland lysates (calculated MW of 30.8kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrinology. 1998 Apr;139(4):2137-45; PMID: 9529003). Recommended concentration: 1-3µg/ml.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 25713511, "intro": "This antibody has been successfully used in IHC in Human:", "title": "Neuropeptide co-expression in hypothalamic kisspeptin neurons of laboratory animals and the human.", "author": "Skrapits K, Borsay BÁ, Herczeg L, Ciofi P, Liposits Z, Hrabovszky E.", "journal": "Front Neurosci. 2015 Feb 10;9:29."}]