Goat Anti-Proenkephalin (mouse) Antibody

$423.00

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SKU: EB08196
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
proenkephalin
Accession ID
NP_001002927.1
Blocking Peptide
EBP08196
Immunogen
Peptide with sequence C-YKDSSKQDESH, from the internal region of the protein sequence according to NP_001002927.1.
Peptide Sequence
C-YKDSSKQDESH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat
Reactive Species
Mouse, Rat
Mouse Gene ID
18619
Rat Gene ID
29237
Product Grade
IHC Results
Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus. Recommended concentration, 0.02-0.05µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa according to Mouse NP_001002927.1 and 30.9kDa according to Rat NP_058835.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC