Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5|EST422562|ABCB5beta|ABCB5alpha|ATP-binding cassette, sub-family B (MDR/TAP), member 5 | |
Usage Summary Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_848654.3; NP_001157413.1 | |
Blocking Peptide EBP08211 | |
Immunogen Peptide with sequence C-QTQHRNTSKKAQ , from the internal region of the protein sequence according to NP_848654.3; NP_001157413.1. | |
Product Comments This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1) | |
Peptide Sequence C-QTQHRNTSKKAQ | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human, Mouse | |
Human Gene ID 340273 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 80kDa band observed in lysates of cell line A431(calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. | |
Application Type Pep-ELISA, WB, FC |
Goat Anti-ABCB5 Antibody
$423.00
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Selected References [{"pmid": 20682318, "intro": "This antibody (previous batch) has been successfully used in Flow Cytometry on Human:", "title": "Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance.", "author": "Cheung ST, Cheung PF, Cheng CK, Wong NC, Fan ST.", "journal": "Gastroenterology. 2011 Jan;140(1):344-55."}, {"pmid": 39267923, "intro": "This antibody has been successfully used in Flow Cytometry on Mouse:", "title": "The uniqueness of ABCB5 as a full transporter ABCB5FL and a half-transporter-like ABCB5?", "author": "Louise Gerard, Jean-Pierre Gillet", "journal": "Cancer Drug Resist. 2024 Aug 7:7:29."}] |