Goat Anti-GNAT3 Antibody

$423.00

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SKU: EB08224
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
gustducin alpha-3|GDCA|tcag7.1053|guanine nucleotide binding protein, alpha transducing 3|GNAT3
Accession ID
NP_001095856.1
Blocking Peptide
EBP08224
Immunogen
Peptide with sequence C-KNQFLDLNLKKEDKE, from the internal region of the protein sequence according to NP_001095856.1.
Peptide Sequence
C-KNQFLDLNLKKEDKE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human
Human Gene ID
346562
Mouse Gene ID
242851
Rat Gene ID
286924
Product Grade
IHC Results
In paraffin embedded Human Small Intestine shows staining of endocrine cells amongst the enterocytes. Recommended concentration: 3-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 27kDa band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.4kDa according to NP_001095856.1. The 27kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC