Goat Anti-PPP2R3A Antibody

$423.00

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SKU: EB08233
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
PPP2R3A|protein phosphatase 2 (formerly 2A), regulatory subunit B\1\1, alpha|PPP2R3|PR130|PR72|PP2A, subunit B, B\1\1-PR72/PR130|PP2A, subunit B, B72/B130 isoforms|PP2A, subunit B, R3 isoform|Serine/threonine protein phosphatase 2A, 72/130 kDa regul
Accession ID
NP_002709.2; NP_871626.1
Blocking Peptide
EBP08233
Immunogen
Peptide with sequence C-QKDVENDGPEPSD, from the internal region of the protein sequence according to NP_002709.2; NP_871626.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_002709.2; NP_871626.1).
Peptide Sequence
C-QKDVENDGPEPSD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
5523
Product Grade
IHC Results
Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments gave an approx 80kDa band in Human Skeletal Muscle lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 130kDa according to NP_002709.2 and of 61.1kDa according to NP_871626.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC