Goat Anti-AIM2 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB09874
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
AIM2|PYHIN4|absent in melanoma 2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_004824.1; NP_001335176.1
Blocking Peptide
EBP09874
Immunogen
Peptide with sequence C-GEKLQLTSGVHSTIK, from the C Terminus of the protein sequence according to NP_004824.1; NP_001335176.1.
Peptide Sequence
C-GEKLQLTSGVHSTIK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
9447
Product Grade
IHC Results
In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration: 4-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 37kDa band observed in lysates of cell line HeLa (calculated MW of 39.0kDa according to Human NP_004824.1). Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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