Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names CDK5 regulatory subunit associated protein 1-like 1|FLJ20342|FLJ46705|MGC75469 | |
Accession ID NP_060244.2 | |
Blocking Peptide EBP08288 | |
Immunogen Peptide with sequence C-QVKKQRTKDLSR, from the internal region of the protein sequence according to NP_060244.2. | |
Peptide Sequence C-QVKKQRTKDLSR | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Human Gene ID 54901 | |
Mouse Gene ID 68916 | |
Rat Gene ID 361243 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot This antibody has been described in a publication and it has been successfully used on Human islets and pancreatic insulinoma INS-1 and MIN6 cells . Preliminary experiments gave an approx. 39kDa band in Human Brain (Cerebellum) lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 65.1kDa according to NP_060244.2. The 39kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? | |
Application Type Pep-ELISA, WB |
Goat Anti-CDKAL1 Antibody
$423.00
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Selected References [{"pmid": 23048041, "intro": "", "title": "CDK5 regulatory subunit-associated protein 1-like 1\r\n(CDKAL1) is a tail-anchored protein in the endoplasmic reticulum (ER) of insulinoma cells.", "author": "Brambillasca S, Altkrueger A, Colombo SF, Friederich A, Eickelmann P, Mark M, Borgese N, Solimena M.", "journal": "J Biol Chem. 2012 Dec 7;287(50):41808-19."}] |