Goat Anti-EXO1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

EXO1|exonuclease 1|HEX1|hExoI|rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1

NP_003677.3; NP_006018.3; NP_569082.1

EBP08306

Peptide with sequence C-HRNYSPRPESGT, from the internal region of the protein sequence according to NP_003677.3; NP_006018.3; NP_569082.1.

This antibody is expected to recognise all three reported isoforms (NP_003677.3; NP_006018.3 and NP_569082.1)

C-HRNYSPRPESGT

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

9156

Antibody detection limit dilution 1:64000.

Approx 90kDa band observed in lysates in cell lines HEK293 and A549 (calculated MW of 89.1kDa according to NP_003677.3). Recommended concentration: 0.2-0.5µg/ml. An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, WB