Goat Anti-NRAS Antibody

$423.00

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SKU: EB08365
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
v-ras neuroblastoma RAS viral oncogene homolog|N-ras protein part 4|NRAS1|N-ras|neuroblastoma RAS viral (v-ras) oncogene homolog|NRAS
Usage Summary
Immunofluorescence: A customer obtained weak cytoplasmic staining in MCF7 cells at 10µg/ml.
Accession ID
NP_002515.1
Blocking Peptide
EBP08365
Immunogen
Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.
Peptide Sequence
C-RMKKLNSSDDGTQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human
Human Gene ID
4893
Mouse Gene ID
18176
Rat Gene ID
24605
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx. 21-22kDa band observed in lysates of cell line MCF7 (calculated MW of 21.2kDa according to NP_002515.1). Recommended concentration: 0.3-1µg/ml. Primary incubation1 hour at room temperature.
Application Type
Pep-ELISA, WB