Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names adiponutrin|Adpn|patatin-like phospholipase domain containing 3|Pnpla3 | |
Accession ID NP_473429.2 | |
Blocking Peptide EBP08402 | |
Immunogen Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2. | |
Product Comments This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2. | |
Peptide Sequence EHDICPKVKSTN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat | |
Reactive Species Human, Mouse | |
Human Gene ID 80339 | |
Mouse Gene ID 116939 | |
Rat Gene ID 362972 | |
Product Grade  | |
IHC Results Paraffin embedded Human Kidney. Recommended concentration: 8µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx 45kDa band observed in Mouse Adrenal Gland lysates, and also in preliminary testing of Mouse and Human Kidney and Rat Adrenal Gland lysate (calculated MW of 42.6Da according to Mouse NP_473429.2). Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-Pnpla3 / Adiponutrin (mouse) Antibody
$423.00
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Selected References [{"pmid": 31238009, "intro": "This antibody (previous batch) has been successfully used in Western blot on Rat:", "title": "Decreased liver triglyceride content in adult rats exposed to protein restriction during gestation and lactation: Roles of hepatic lipogenesis and lipid utilization in muscle and adipose tissue", "author": "Mendez-Garcia C, Trini A, Browne V, Kochansky CJ, Pontiggia L, D'mello AP", "journal": "Can J Physiol Pharmacol. 2019 Oct;97(10):952-962"}, {"pmid": 21673048, "intro": "This antibody (previous batch) has been successfully used in Western blot on Rat:", "title": "Sex-dependent hepatic transcripts and metabolites in the development of glucose intolerance and insulin resistance in Zucker diabetic\r\nfatty rats.", "author": "Gustavsson C, Soga T, Wahlström E, Vesterlund M, Azimi A, Norstedt G, Tollet-Egnell P.", "journal": "J Mol Endocrinol. 2011 Aug 25;47(2):129-43."}, {"pmid": 37524709, "intro": "This antibody (previous batch) has been successfully used in WB on Mouse:", "title": "Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34", "author": "Ryuta Sugihara, Manabu Taneike, Tomokazu Murakawa, Takahito Tamai, Hiromichi Ueda, Rika Kitazume-Taneike, Takafumi Oka, Yasuhiro Akazawa, Hiroki Nishida, Kentaro Mine, Ayana Hioki, Jumpei Omi, Shigemiki Omiya, Junken Aoki, Kazutaka Ikeda, Kazuhiko Nishida, Makoto Arita, Osamu Yamaguchi, Yasushi Sakata, Kinya Otsu", "journal": "Nat Commun. 2023 Jul 31;14(1):4494."}, {"pmid": 22560221, "intro": "This antibody (previous batch) has been successfully used in Western blot on Mouse:", "title": "Adiponutrin functions as a nutritionally regulated\r\nlysophosphatidic acid acyltransferase.", "author": "Kumari M, Schoiswohl G, Chitraju C, Paar M, Cornaciu I, Rangrez AY, Wongsiriroj N, Nagy HM, Ivanova PT, Scott SA, Knittelfelder O, Rechberger GN, Birner-Gruenberger R, Eder S, Brown HA, Haemmerle G, Oberer M, Lass A, Kershaw EE, Zimmermann R, Zechner R.", "journal": "Cell Metab. 2012 May 2;15(5):691-702."}] |