Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names SKase|SK1|SPHK|sphingosine kinase 1|SPHK1 | |
Usage Summary Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected. | |
Accession ID NP_068807.2; NP_892010.2; NP_001136073.1 | |
Blocking Peptide EBP08409 | |
Immunogen Peptide with sequence C-DVDLESEKYRRLGE, from the internal region of the protein sequence according to NP_068807.2; NP_892010.2; NP_001136073.1. | |
Product Comments This antibody is expected to recognise all three reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). Reported variants represent identical protein (NP_001136073.1; NP_001136074.1). | |
Peptide Sequence C-DVDLESEKYRRLGE | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow | |
Reactive Species Human | |
Human Gene ID 8877 | |
Mouse Gene ID 20698 | |
Rat Gene ID 170897 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3µg/ml. | |
Application Type Pep-ELISA, WB, IP |
Goat Anti-SPHK1 Antibody
$423.00
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