Goat Anti-Ankyrin 1 / ANK1 Antibody

$255.00

Category: Primary Antibodies
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SKU: EB08509
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ankyrin-R|ankyrin-1, erythrocytic|ankyrin 1|SPH2|SPH1|ANK|ankyrin 1, erythrocytic|ANK1
Accession ID
NP_000028.3; NP_065208.2; NP_065209.2; NP_065210.2; NP_065211.2; NP_065213.2
Blocking Peptide
EBP08509
Immunogen
Peptide with sequence C-QIVKRASLKRGKQ, from the C Terminus of the protein sequence according to NP_000028.3; NP_065208.2; NP_065209.2; NP_065210.2; NP_065211.2; NP_065213.2.
Product Comments
This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).
Peptide Sequence
C-QIVKRASLKRGKQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Human Gene ID
286
Mouse Gene ID
11733
Rat Gene ID
306570
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kDa according to NP_065211.2. The 19-22 kDa bands were successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA

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