Goat Anti-XBP1 / TREB5 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB08557
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
X-box-binding protein-1|OTTHUMP00000028682|XBP2|X-box binding protein 1|TREB5|XBP1
Usage Summary
Immunofluorescence: Expression of the protein seen in the endoplasmic reticulum, cytoplasm and nucleus of HeLa and MCF7 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_001073007.1
Blocking Peptide
EBP08557
Immunogen
Peptide with sequence C-NHSWEDTFANELFPQ, from the internal region of the protein sequence according to NP_001073007.1.
Product Comments
This antibody is expected to recognise isoform XBP1(S) (NP_001073007.1) only, The epitope for EB08557 is aa358-372.
Peptide Sequence
C-NHSWEDTFANELFPQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human
Human Gene ID
7494
Mouse Gene ID
22433
Product Grade
IHC Results
Paraffin embedded Human Breast. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx 38kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 40.1kDa according to NP_001073007.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour.at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC

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