Goat Anti-ABCA8 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

ABCA8|ATP-binding cassette, sub-family A (ABC1), member 8|KIAA0822|MGC163152|ATP-binding cassette, sub-family A member 8

NP_009099.1

EBP08618

Peptide with sequence C-KEQELGDFEEDFD, from the C Terminus of the protein sequence according to NP_009099.1.

This antibody is expected to cross-react with the highly similar Human ABCA9, since the immunizing peptide differs from ABCA9 with only one residue (F8).

C-KEQELGDFEEDFD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

10351

Antibody detection limit dilution 1:4000.

Preliminary experiments gave an approx. 50-55kDa band in Human Brain (Cerebellum, Cerebral Cortex, Frontal Cortex), Heart, Skeletal Muscle and Testis lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 179kDa according to NP_009099.1. The 50-55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA