Goat Anti-RELMbeta Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

RETNLB|resistin like beta|FIZZ1|FIZZ2|HXCP2|RELM-beta|RELMb|RELMbeta|XCP2|C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2|colon and small intestine-specific cysteine-rich protein|cysteine-rich secreted A12-alpha-like protein 1|found in inflammatory zone 1

NP_115968.1

EBP08633

Peptide with sequence C-DSVMDKKIKDVLNS, from the internal region of the protein sequence according to NP_115968.1.

C-DSVMDKKIKDVLNS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

84666

In paraffin embedded Human Colon shows textured staining of cytoplasm in enterocytes. Recommended concentration, 2-4µg/ml.

Antibody detection limit dilution 1:128000.

Preliminary experiments gave an approx 35kDa band in Human Ileum and Duodenum lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to NP_115968.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC