Goat Anti-USP28 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

ubiquitin specific protease 28|KIAA1515|ubiquitin specific peptidase 28|USP28

NP_065937.1

EBP08663

Peptide with sequence C-SVELKHYIQEDN, from the internal region of the protein sequence according to NP_065937.1.

C-SVELKHYIQEDN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Cow

Human

57646

In paraffin embedded Human Testis shows acrosomal staining in spermatids. Recommended concentration, 2.5-3.8µg/ml.

Antibody detection limit dilution 1:64000.

Preliminary experiments gave bands at approx 150kDa and 50kDa in Human Bone Marrow lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 122kDa according to NP_065937.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC