Goat Anti-IRAK3 (mouse) Antibody

$423.00

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SKU: EB08674
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
interleukin-1 receptor-associated kinase M|IRAKM|IRAK-M|ASRT5|interleukin-1 receptor-associated kinase 3|IRAK3
Accession ID
NP_082955.2
Blocking Peptide
EBP08674
Immunogen
Peptide with sequence C-GHSYGSKPMEKR, from the internal region (near the C Terminus) of the protein sequence according to NP_082955.2.
Peptide Sequence
C-GHSYGSKPMEKR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat
Reactive Species
Mouse, Rat
Mouse Gene ID
73914
Rat Gene ID
314870
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 65-70kDa band observed in Rat Liver lysates, and in preliminary testing of Rat Adrenal Gland lysate (calculated MW of 69.0kDa according to Rat, NP_001101571.1 and 67.8kDa according to Mouse NP_082955.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.

A customer detected a band at approx. 70kDa in Mouse Bone Marrow lysates, which was not present in the knock-out mouse.

Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 36757800, "intro": "This antibody has been successfully used in the following paper:", "title": "IL-1 receptor-associated kinase-3 acts as an immune checkpoint in myeloid cells to limit cancer immunotherapy", "author": "Gurcan Tunali, Marta Rúbies Bedós, Divya Nagarajan, Patrik Fridh, Irineos Papakyriacou, Yumeng Mao", "journal": "J Clin Invest. 2023 Apr 3;133(7):e161084."}]