Goat Anti-ABCB10 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

ABCB10|ATP-binding cassette, sub-family B (MDR/TAP), member 10|EST20237|M-ABC2|MTABC2|ATP-binding cassette, sub-family B, member 10|ABC-me

NP_036221.2

EBP08701

Peptide with sequence C-KPNGIYRKLMNKQ, from the C Terminus of the protein sequence according to NP_036221.2.

C-KPNGIYRKLMNKQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat

23456

56199

361439

Antibody detection limit dilution 1:64000.

Preliminary experiments gave bands at approx. 90kDa and 50kDa in Human Bone Marrow lysates after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 79.1kDa according to NP_036221.2 and given the expected mature size of ~65kDa according to Graf et al. (PMID: 15215243). Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA