Goat Anti-APOL4 (C Terminus) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08768
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
apolipoprotein L4|APOL4|apolipoprotein L, 4|APOL-IV|APOLIV|OTTHUMP00000028937|apolipoprotein L-IV
Accession ID
NP_085146.2; NP_663693.1
Blocking Peptide
EBP08768
Immunogen
Peptide with sequence C-ENLNELTHIHQSLK, from the C Terminus of the protein sequence according to NP_085146.2; NP_663693.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_085146.2 and NP_663693.1).
Peptide Sequence
C-ENLNELTHIHQSLK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
80832
Product Grade
IHC Results
Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx. 37kDa band observed in Human Placenta lysates (calculated MW of 38.7kDa according to NP_085146.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Two additional bands of unknown identity were also consistently observed at 50kDa and at 70kDa. The band at 50kDa was successfully blocked by incubation with the immunising peptide. The other band at 70kDa however was not blocked by the immunizing peptide and it is therefore a non-specific signal.
Application Type
Pep-ELISA, WB, IHC

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