Goat Anti-APOB Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08792
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
apolipoprotein B|apoB-100|FLDB|apolipoprotein B (including Ag(x) antigen)|APOB
Accession ID
NP_000375.3
Blocking Peptide
EBP08792
Immunogen
Peptide with sequence C-TDLHLRYQKDKK, from the internal region of the protein sequence according to NP_000375.3.
Product Comments
This antibody only recognises ApoB-100 and not the cleaved chain ApoB-48.
Peptide Sequence
C-TDLHLRYQKDKK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
338
Product Grade
IHC Results
Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 2µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Customer data showed a band corresponding to the exopected size of ApoB-100 in Human serum samples from five different individuals (calculated Mwt of 515kDa according to NP_000375.3). The smalller bands shown are believed to be degradation products of ApoB-100 (not ApoB-48). Recommended concentration: 0.3-1µg/ml.
Application Type
Pep-ELISA, WB, IHC

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