Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names Mcp10|Mast cell protease 10|Chymase 2, mast cell|Cma2 | |
Accession ID NP_001019885.1; NP_034912.3 | |
Blocking Peptide EBP08817 | |
Immunogen Peptide with sequence CKIFKHYKDSLQ, from the intenal region of the protein sequence according to NP_001019885.1; NP_034912.3. | |
Product Comments Mcp 10 and Mcp 9 (GeneID 545055) are almost identical in their protein sequence. No cross-reactivity expected with the similar Mcp4. | |
Peptide Sequence CKIFKHYKDSLQ | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Mouse | |
Mouse Gene ID 17232 | |
Product Grade ![]() | |
ELISA Detection Limit Antibody detection limit dilution 1:2000. | |
Western Blot Preliminary experiments gave an approx 49kDa band in Human Skeletal Muscle lysates after 0.02µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 26.9kDa according to NP_001019885.1 and NP_034912.3. The 49kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? | |
Application Type Pep-ELISA |
Goat Anti-Mcp10 / Mcp9 Antibody
$255.00
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