Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names purinoceptor P2X4|purinergic receptor P2X4|purinergic receptor P2X ligand-gated ion channel 4|P2X receptor, subunit 4|P2X purinoceptor 4|ATP-gated cation channel protein|ATP receptor|purinergic receptor P2X, ligand-gated ion channel, 4|P2RX4|P2X4R|P2X4 | |
Usage Summary Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_002551.2; NP_001243725.1; NP_001248326.1 | |
Blocking Peptide EBP08830 | |
Immunogen Peptide with sequence C-YREKKYKYVEDYEQ, from the C Terminus of the protein sequence according to NP_002551.2; NP_001243725.1; NP_001248326.1. | |
Peptide Sequence C-YREKKYKYVEDYEQ | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow | |
Reactive Species Human | |
Human Gene ID 5025 | |
Mouse Gene ID 18438 | |
Rat Gene ID 29659 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 60kDa band observed in Human Kidney lysates and in preliminary testing of Human Liver lysate (calculated MW of 43.4kDa according to NP_002551.2).This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, FC |
Goat Anti-P2X4 / P2X4R Antibody
$423.00
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Selected References [{"pmid": 20025063, "intro": "This antibody (previous batch) has been successfully used in the following paper:", "title": "Role of mast cell activation in inducing microglial cells to release neurotrophin.", "author": "Yuan H, Zhu X, Zhou S, Chen Q, Zhu X, Ma X, He X, Tian M, Shi X.", "journal": "J Neurosci Res. 2010 May 1;88(6):1348-54."}, {"pmid": 21106936, "intro": "This antibody (previous batch) has been successfully used in the following paper:", "title": "Regenerative protein thymosin beta-4 is a novel regulator of purinergic signaling.", "author": "Freeman KW, Bowman BR, Zetter BR.", "journal": "FASEB J. 2011 Mar;25(3):907-15."}] |