Goat Anti-CTLA + CTLB Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08878
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
LCB|clathrin, light chain (Lcb)|clathrin, light polypeptide A|clathrin, light polypeptide (Lca)|LCA|clathrin, light chain (Lca)|CTLB|CLTA
Accession ID
NP_001824.1; NP_009027.1; NP_001070145.1; NP_001825.1; NP_009028.1
Blocking Peptide
EBP08878
Immunogen
Peptide with sequence CDFNPKSSKQAKD, from the internal region of the protein sequence according to NP_001824.1; NP_009027.1; NP_001070145.1; NP_001825.1; NP_009028.1.
Product Comments
This antibody is expected to recognize all reported isoforms of light chains a and b.
Peptide Sequence
CDFNPKSSKQAKD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
1211, 1212
Mouse Gene ID
12757
Rat Gene ID
83800
Product Grade
IHC Results
Paraffin embedded Human Spleen. Recommended concentration: 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx. 35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC

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