Goat Anti-SEPT2 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

neural precursor cell expressed, developmentally down-regulated 5|hNedd5|Pnutl3|NEDD5|KIAA0158|DIFF6|septin 2|SEPT2

Immunofluorescence: Strong expression of the protein seen in HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_001008491.1; NP_001269901.1; NP_001269902.1; NP_001307958.1; NP_001336231.1

EBP08931

Peptide with sequence SKQQPTQFINPET-C, from the N terminus of the protein sequence according to NP_001008491.1; NP_001269901.1; NP_001269902.1; NP_001307958.1; NP_001336231.1.

This antibody is expected to recognise::NP_001008491.1 (iso a); NP_001269901.1 (iso b); NP_001269902.1 (iso c); NP_001307958.1; (iso d) NP_001336231.1 (iso f).

SKQQPTQFINPET-C

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat

Human, Mouse, Rat

4735

18000

117515

Paraffin embedded Human Breast, Tonsil and Brain (Cortex). Recommended concentration: 2.5µg/ml.

Antibody detection limit dilution 1:32000.

Approx. 40kDa band observed in lysates of cell lines HepG2, Jurkat, HeLa and KNRK, and in Rat Testis lysates, and approx. 42kDa in lysates of cell lines K562 and NIH3T3, and in Human and Mouse Testis lysates (calculated MW of 41.5kDa according to Human NP_001008491.1, Mouse NP_001153189.1 and Rat NP_476489.1). Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, IF, FC