Goat Anti-SEPT6 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB08932
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
septin 2|SEPT2|SEP2|MGC20339|MGC16619|KIAA0128|septin 6|SEPT6
Usage Summary
Immunofluorescence: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_665798.1; NP_055944.2; NP_665801.1
Blocking Peptide
EBP08932
Immunogen
Peptide with sequence C-DEVNAFKQRKTA, from the internal region of the protein sequence according to NP_665798.1; NP_055944.2; NP_665801.1.
Product Comments
This antibody is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein.
Peptide Sequence
C-DEVNAFKQRKTA
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Cow
Reactive Species
Human
Human Gene ID
23157
Product Grade
IHC Results
In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration: 3-5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx 50kDa band observed in Human Testis, Duodenum, Kidney and Tonsil lysates, and in lysates of cell lines Daudi, Jurkat and MOLT-4 (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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