Goat Anti-Endothelial lipase Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

lipoprotein lipase H|endothelial cell-derived lipase|PRO719|EL|EDL|LIPG|lipase, endothelial|endothelial lipase

NP_006024.1

EBP08961

Peptide with sequence C-RFNLRTSKDPEHEG, from the internal region of the protein sequence according to NP_006024.1.

C-RFNLRTSKDPEHEG

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

9388

Antibody detection limit dilution 1:16000.

Approx. 70kDa band observed in Human Liver lysates (calculated MW of 56.8kDa according to NP_006024.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gauster et al , J Clin Endocrinol Metab. 2007 Jun;92(6):2256-63. Epub 2007 Mar 13.; PMID: 17356047). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, WB