Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names BAG4|BCL2-associated athanogene 4|BAG-4|SODD|BAG-family molecular chaperone regulator-4|silencer of death domains | |
Accession ID NP_004865.1 | |
Blocking Peptide EBP09032 | |
Immunogen Peptide with sequence C-GNATSDHPNNQDQSS, from the internal region of the protein sequence according to NP_004865.1. | |
Peptide Sequence C-GNATSDHPNNQDQSS | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 9530 | |
Product Grade ![]() | |
IHC Results Paraffin embedded Human Placenta. Recommended concentration: 3.75µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx. 60kDa band observed in cell lines HEK293 and HeLa lysates (calculated MW of 49.6kDa according to NP_004865.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Takada et al, Mol Cell Biol. 2003 Jun;23(11):4026-33.; PMID: 12748303). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-SODD Antibody
$423.00
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