Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names OTTHUMP00000018394|BRICHOS domain containing 2B|FBD|E3-16|E25B|BRICD2B|BRI2|BRI|ABRI|integral membrane protein 2B|ITM2B | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_068839.1 | |
Blocking Peptide EBP09138 | |
Immunogen Peptide with sequence C-HDKETYKLQRRETIK, from the internal region of the protein sequence according to NP_068839.1. | |
Peptide Sequence C-HDKETYKLQRRETIK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Cow | |
Reactive Species Human | |
Human Gene ID 9445 | |
Mouse Gene ID 16432 | |
Rat Gene ID 290364 | |
Product Grade  | |
IHC Results Paraffin embedded Human Small Intestine. Recommended concentration: 2.5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx. 30-35kDa band observed in HepG2 cell lysates (calculated MW of 30.3kDa according to NP_068839.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Brain for this particular batch. | |
Application Type Pep-ELISA, WB, IHC, IF, FC |
Goat Anti-ITM2B Antibody
$464.00
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