Goat Anti-DGCR8 / Pasha Antibody

$255.00

Category: Primary Antibodies
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SKU: EB09141
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
DGCR8|DiGeorge syndrome critical region gene 8|C22orf12|DGCRK6|Gy1|pasha
Accession ID
NP_073557.3
Blocking Peptide
EBP09141
Immunogen
Peptide with sequence C-KRFDFEQVTVKKFRT, from the internal region of the protein sequence according to NP_073557.3.
Peptide Sequence
C-KRFDFEQVTVKKFRT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Human Gene ID
54487
Mouse Gene ID
94223
Rat Gene ID
287954
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx. 75kDa band in Human Brain (amygdala) lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 86.0kDa according to NP_073557.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA

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