Goat Anti-TRPC7 Antibody

$423.00

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SKU: EB09257
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
putative capacitative calcium channel|likley ortholog of mouse transient receptor potential cation channel, subfamily C, member 7|TRP7|transient receptor potential cation channel, subfamily C, member 7|TRPC7
Usage Summary
Immunoprecipitation: This antibody has been successfully used in IP on lysates of Chicken Embryo Heart (Sabourin et al,; PMID: 21672930).
Accession ID
NP_065122.1; NP_001161049.1; NP_001161048.1; NP_001363830.1
Blocking Peptide
EBP09257
Immunogen
Peptide with sequence KLCKSKAKSCEND, from the internal region of the protein sequence according to NP_065122.1; NP_001161049.1; NP_001161048.1; NP_001363830.1.
Product Comments
This antibody is expected to cross-react with isoform 1, 2 and 3 (NP_065122.1; NP_001161049.1; NP_001161048.1).
Peptide Sequence
KLCKSKAKSCEND
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Chicken, Mouse
Human Gene ID
57113
Mouse Gene ID
26946
Rat Gene ID
282822
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
A 95kDa band was observed in Chicken Embryo Heart atrial and ventricular myocyte lysates (Sabourin et al,; PMID 21672930). This antibody has been successfully used in WB on Mouse (Murtaza et al.; PMID: 32882106).
Application Type
Pep-ELISA, WB, IP
Selected References
[{"pmid": 21672930, "intro": "This antibody has been successfully used in WB and IP on Chicken:", "title": "A key role of TRPC channels in the regulation \r\nof electromechanical activity of the developing heart.", "author": "Sabourin J, Robin E, Raddatz E.", "journal": "Cardiovasc Res. 2011 Jun 13. [Epub ahead of print]"}, {"pmid": 22692208, "intro": "This antibody has been successfully used in WB on Chicken:", "title": "Activation of Transient Receptor Potential Canonical 3 (TRPC3)-mediated Ca2+ Entry by A1 Adenosine Receptor in Cardiomyocytes Disturbs Atrioventricular Conduction.", "author": "Sabourin J, Antigny F, Robin E, Frieden M, Raddatz E.", "journal": "J Biol Chem. 2012 Aug 3;287(32):26688-701."}, {"pmid": 32882106, "intro": "This antibody has been successfully used in WB on Mouse:", "title": "Implication of TRPC3 channel in gustatory perception of dietary lipids", "author": "Babar Murtaza, Aziz Hichami, Amira S Khan, Jiri Plesnik, Omar Sery, Alexander Dietrich, Lutz Birnbaumer, Naim A Khan", "journal": "Acta Physiol (Oxf). 2020 Sep 3;e13554.  doi: 10.1111/apha.13554."}]