Goat Anti-DLL1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB09285
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
delta-like 1 protein|delta-like 1|delta (Drosophila)-like 1|OTTHUMP00000017690|Delta|DL1|DELTA1|delta-like 1 (Drosophila)|DLL1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_005609.3
Blocking Peptide
EBP09285
Immunogen
Peptide with sequence C-ATQRHLTVGEEWSQD, from the internal region of the protein sequence according to NP_005609.3.
Peptide Sequence
C-ATQRHLTVGEEWSQD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human, Rat
Human Gene ID
28514
Mouse Gene ID
13388
Rat Gene ID
84010
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 75kDa band observed in Mouse Heart and Rat Pancreas lysates (calculated MW of 78.5kDa according to Mouse NP_031891.2 and 77.4kDa according to Rat NP_114452.1). An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration:0.3-7µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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