Goat Anti-LIMP2 / SCARB2 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

scavenger receptor class B member 2|LIMP II|CD36 antigen-like 2|85 kDa lysosomal membrane sialoglycoprotein|LIMP-2|LGP85|EPM4|lysosomal integral membrane protein II|CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II)|85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2|SR-BII|LIMPII|HLGP85|CD36L2|AMRF|scavenger receptor class B, member 2|SCARB2

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

NP_005497.1

EBP09295

Peptide with sequence C-NKANIQFGDNGTTIS, from the internal region of the protein sequence according to NP_005497.1.

This antibody is expected to recognize reported isoform 1 (NP_005497.1) only.

C-NKANIQFGDNGTTIS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

950

12492

In paraffin embedded Human Cerebral Cortex shows lysosomal staining. Recommended concentration: 3-5µg/ml.

Antibody detection limit dilution 1:4000.

Approx. 80kDa band observed in Human Cerebral Cortex lysates and in preliminary testing of Human Thyroid lysate and U251 cell lysate (calculated MW of 54.3kDa according to NP_005497.1). This molecular weight is observed in the literature (Fujita et al, 1992, PMID: 1374238) and by other commercial sources. Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature. This antibody has been successfully used in WB on Human: Murphy KE et al. (2014) PMID: 24477431.

Pep-ELISA, WB, IHC, IF, FC