Goat Anti-CD11A / integrin alpha L Antibody

$464.00

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SKU: EB09493
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
lymphocyte function-associated antigen 1|integrin gene promoter|integrin alpha L|antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide|LFA-1 alpha|LFA1A|LFA-1|CD11A|alpha polypeptide)|integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1|ITGAL
Usage Summary

Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002200.2; NP_001107852.1
Blocking Peptide
EBP09493
Immunogen
Peptide with sequence C-YQVRIQPSIHDHN, from the internal region of the protein sequence according to NP_002200.2; NP_001107852.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).
Peptide Sequence
C-YQVRIQPSIHDHN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
3683
Product Grade
IHC Results
In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 2-4µg/ml. Paraffin embedded Human Spleen. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx. 200kDa band observed in lysates of cell line THP-1 (calculated MW of 129kDa according to NP_002200.2). This molecular weight is observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.

Application Type
Pep-ELISA, WB, IHC, FC