Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names lymphocyte function-associated antigen 1|integrin gene promoter|integrin alpha L|antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide|LFA-1 alpha|LFA1A|LFA-1|CD11A|alpha polypeptide)|integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1|ITGAL | |
Usage Summary Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_002200.2; NP_001107852.1 | |
Blocking Peptide EBP09493 | |
Immunogen Peptide with sequence C-YQVRIQPSIHDHN, from the internal region of the protein sequence according to NP_002200.2; NP_001107852.1. | |
Product Comments This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1). | |
Peptide Sequence C-YQVRIQPSIHDHN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 3683 | |
Product Grade ![]() | |
IHC Results In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 2-4µg/ml. Paraffin embedded Human Spleen. Recommended concentration: 3.75µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx. 200kDa band observed in lysates of cell line THP-1 (calculated MW of 129kDa according to NP_002200.2). This molecular weight is observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability. | |
Application Type Pep-ELISA, WB, IHC, FC |
Goat Anti-CD11A / integrin alpha L Antibody
$464.00
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