Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names major histocompatibility complex class I antigen-binding protein p88|P90|IP90|FLJ26570|CNX|calnexin|CANX | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of U251 cells and in the endoplasmic reticulum and plasma membranes of LNCaP cells. Recommended concentration: 10µg/ml. | |
Accession ID NP_001737.1 | |
Blocking Peptide EBP09525 | |
Immunogen Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1. | |
Product Comments Reported variants represent identical protein (NP_001019820.1, NP_001737.1). | |
Peptide Sequence C-SKTPELNLDQFHDKT | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human, Rat | |
Human Gene ID 821 | |
Mouse Gene ID 12330 | |
Rat Gene ID 29144 | |
Product Grade  | |
IHC Results Paraffin embedded Human Cortex. Recommended concentration: 6µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 90-100kDa band observed in Human Cerebellum and Rat Brain lysates and in lysates of cell lines LNCaP and U251 (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.3kDa according to Rat NP_742005.1). This molecular weight is routinely observed by other sources, Recommended concentration: 0.1-0.5/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IHC, IF |
Goat Anti-Calnexin Antibody
$464.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |