Goat Anti-Connexin 30 / GJB6 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

GJB6|gap junction protein, beta 6, 30kDa|CX30|DFNA3|ED2|EDH|HED|OTTHUMP00000018096|OTTHUMP00000176870|OTTHUMP00000176871|OTTHUMP00000176872|connexin 30|ectodermal dysplasia 2, hidrotic (Clouston syndrome)|gap junction protein, beta 6|gap junction protein, beta 6 (connexin 30)

NP_006774.2

EBP09671

Peptide with sequence C-RSKRAQTQKNHPNH, from the internal region of the protein sequence according to NP_006774.2.

Reported variants represent identical protein: NP_006774.2, NP_001103691.1, NP_001103689.1, NP_001103690.1

C-RSKRAQTQKNHPNH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Cow

10804

14623

84403

Antibody detection limit dilution 1:16000.

Preliminary experiments gave an approx 14kDa band in Human Brain (Cerebellum) and Rat Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.4kDa according to NP_006774.2. The 14kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA