Goat Anti-PRDM1 / MEL1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

positive regulatory domain I-binding factor 1|beta-interferon gene positive-regulatory domain I binding factor|PRDI-binding factor-1|PR-domain zinc finger protein 1|OTTHUMP00000016918|B-lymphocyte-induced maturation protein 1|PRDI-BF1|MGC118925|MGC118924|MGC118923|MGC118922|BLIMP1|PR domain containing 1, with ZNF domain|PRDM1

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

NP_001189.2; NP_878911.1

EBP09715

Peptide with sequence C-DISDNADRLEDVED, from the internal region of the protein sequence according to NP_001189.2; NP_878911.1.

This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1).

C-DISDNADRLEDVED

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow, Pig

Human

639

12142

309871

Paraffin embedded Human Testis. Recommended concentration: 6µg/ml.

Antibody detection limit dilution 1:1000.

Approx 90kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100kDa in lysates of cell line K562. An additional faint band of approx 70kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8kDa according to NP_001189.2 isoform 1 and 76.8kD according to NP_878911.1 isoform 2). Recommended concentration: 0.1-2g/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, IF, FC