Goat Anti-NDUFS6 Antibody

$423.00

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SKU: EB09788
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)|NDUFS6
Usage Summary
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Accession ID
NP_004544.1
Blocking Peptide
EBP09788
Immunogen
Peptide with sequence C-RIRFVGRQKEVNEN, from the internal region of the protein sequence according to NP_004544.1.
Peptide Sequence
C-RIRFVGRQKEVNEN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Mouse, Rat
Human Gene ID
4726
Mouse Gene ID
407785
Rat Gene ID
29478
Product Grade
IHC Results
Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 15kDa band observed in Human Heart lysates and 14kDa in Mouse and Rat Heart lysates (calculated MW of 13.7kDa according to NP_004544.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]