Goat Anti-Muc5b (mouse) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

2300002I04Rik|A130042M24|AV085033|MUC5|MUC9|mucin 5, subtype B, tracheobronchial|mucin 5b|Muc5b

NP_083077.2

EBP10312

Peptide with sequence CEKEDSSTCQ, from the internal region of the protein sequence according to NP_083077.2.

CEKEDSSTCQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Mouse, Rat

74180

309114

Antibody detection limit dilution 1:4000.

Preliminary experiments gave bands at approx 100kDa and 55kDa in Mouse Lung lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 515kDa according to Mouse NP_083077.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA