Goat Anti-CPLA2-zeta Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

cPLA2-zeta|cytosolic phospholipase A2 zeta|DKFZp666G192|DKFZp781B229|phospholipase A2, group IVF|PLA2G4FZ|PLA2G4F

NP_998765.3

EBP09974

Peptide with sequence C-QTAEEKAFGDFVINR, from the internal region (near C Terminus) of the protein sequence according to NP_998765.3.

C-QTAEEKAFGDFVINR

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Cow

255189

Antibody detection limit dilution 1:16000.

Preliminary experiments gave bands at approx 55-60kDa and 35kDa in Human Placenta lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kDa according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA