Goat Anti-POU5F1B Antibody

$255.00

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SKU: EB10038
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
POU5F1B|POU5FLC8|POU5FLC20|POU5F1P1|POU domain, class 5, transcription factor 1 pseudogene 1|POU domain transcription factor Oct-4|POU class 5 homeobox 1B|POU class 5 homeobox 1 pseudogene 1|POU 5 domain protein|OTF3P1|OTF3C|octamer b|OCT4-pg1
Accession ID
NP_001153014.1
Blocking Peptide
EBP10038
Immunogen
Peptide with sequence C-TPGYGSPHFTALYS, from the internal region (near C Terminus) of the protein sequence according to NP_001153014.1.
Product Comments
This antibody is expected to crossreact to the almost identical OCT4 / POU5F1 (GeneID 5460).
Peptide Sequence
C-TPGYGSPHFTALYS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Human Gene ID
5462, 5460
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.6kDa according to NP_001153014.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA