Goat Anti-Npc1(mouse) Antibody

$255.00

Category: Primary Antibodies
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SKU: EB10096
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
Npc1|spm|sphingomyelinosis|nmf164|niemann-Pick C1 protein|Niemann Pick type C1|lcsd|D18Ertd723e|D18Ertd139e|C85354|A430089E03Rik
Accession ID
NP_032746.2
Blocking Peptide
EBP10096
Immunogen
Peptide with sequence C-TYHTILKTSADYTD, from the internal region of the protein sequence according to NP_032746.2.
Peptide Sequence
C-TYHTILKTSADYTD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse
Mouse Gene ID
18145
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments gave an approx 36kDa band in lysates of cell lines NIH3T3 and NSO after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 142kDa according to NP_032746.2. The 36kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA

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