Goat Anti-DCTN3 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB10446
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
DCTN3|MGC111190|dynactin light chain|dynactin 3, isoform 1|dynactin 3 (p22)|dynactin 3|DCTN-22|DCTN22
Usage Summary

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_009165.1; NP_077324.1
Blocking Peptide
EBP10446
Immunogen
Peptide with sequence C-QVALGNISSKRE, from the internal region of the protein sequence according to NP_009165.1; NP_077324.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).
Peptide Sequence
C-QVALGNISSKRE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Rat
Human Gene ID
11258
Mouse Gene ID
53598
Rat Gene ID
362504
Product Grade
IHC Results
Paraffin embedded Human Kidney. Recommended concentration: 6-8µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 20kDa band observed in Human Liver lysates, and approx. 18kDa in Rat Kidney lysates and in preliminary testing of Mouse Kidney lysate (calculated MW of 19.5kDa according to Human NP_077324.1 and 21.1kDa according to Rat NP_001102129.1. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC

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