Goat Anti-FANCE Antibody

$423.00

Category: Primary Antibodies
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SKU: EB10143
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FANCE|Fanconi anemia, complementation group E|FAE|FACE
Accession ID
NP_068741.1
Blocking Peptide
EBP10143
Immunogen
Peptide with sequence C-EHKSLESLADGGS, from the internal region of the protein sequence according to NP_068741.1.
Peptide Sequence
C-EHKSLESLADGGS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Cow
Reactive Species
Human
Human Gene ID
2178
Product Grade
IHC Results
In paraffin embedded Human Placenta shows nuclear staining in some trophoblasts. Recommended concentration, 3-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 70kDa band in Human Adipose and Uterus lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.7kDa according to NP_068741.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA, IHC

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