Goat Anti-Desmoplakin (aa 1546-1558) Antibody

$255.00

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SKU: EB10152
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
250 kDa paraneoplastic pemphigus antigen|desmoplakin|desmoplakin I|DPI|DSP
Accession ID
NP_004406.2
Blocking Peptide
EBP10152
Immunogen
Peptide with sequence C-SQERTVKDQDITR, from the internal region of the protein sequence according to NP_004406.2.
Product Comments
This antibody is expected to recognize reported isoform I (NP_004406.2) only.
Peptide Sequence
C-SQERTVKDQDITR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Human Gene ID
1832
Mouse Gene ID
109620
Rat Gene ID
306871
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Application Type
Pep-ELISA