Goat Anti-ETFA (aa139-152) Antibody

$423.00

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SKU: EB10173
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
electron transfer flavoprotein alpha-subunit|electron transfer flavoprotein, alpha polypeptide|electron-transfer-flavoprotein, alpha polypeptide|EMA|GA2|glutaric aciduria II|MADD|ETFA
Accession ID
NP_000117.1; NP_001121188.1
Blocking Peptide
EBP10173
Immunogen
Peptide with sequence C-KSPDTFVRTIYAGN, from the internal region of the protein sequence according to NP_000117.1; NP_001121188.1.
Product Comments
This antibody is expected to recognize both isoforms (NP_000117.1; NP_001121188.1). Amino acid numbering in name refers to NP_000117.1 sequence)
Peptide Sequence
C-KSPDTFVRTIYAGN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Pig, Dog, Cow
Reactive Species
Human
Human Gene ID
2108
Mouse Gene ID
110842
Rat Gene ID
300726
Product Grade
IHC Results
Paraffin embedded Human Adrenal Gland and Heart. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 30+35kDa bands observed in Human Colon lysates (calculated MW of 35.1kDa according to NP_000117.1 and of 30.0kDa according to NP_001121188.1. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 27059956, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "The mitochondrial isoform of Shc protein inhibits thiolase and lipid oxidation.", "author": "Tomilov A, Tomilova N, Shan Y, Hagopian K, Bettaieb A, Kim K, Pelicci PG, Haj F, Ramsey J, Cortopassi G.", "journal": "J Biol Chem. 2016 Apr 8."}]