Goat Anti-CIRBP (aa 81-91) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB10235
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
CIRBP|glycine-rich RNA binding protein|Cold-inducible RNA-binding protein|cold inducible RNA-binding protein|cold inducible RNA binding protein|CIRP
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001271.1; NP_001287758.1; NP_001287744.1
Blocking Peptide
EBP10235
Immunogen
Peptide with sequence C-QAGKSSDNRSR, from the internal region of the protein sequence according to NP_001271.1; NP_001287758.1; NP_001287744.1.
Peptide Sequence
C-QAGKSSDNRSR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Cow
Reactive Species
Human
Human Gene ID
1153
Mouse Gene ID
12696
Rat Gene ID
81825
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx. 19kDa band observed in nuclear lysates of cell line MCF7 and in preliminary testing of Human Ovary lysate (calculated MW of 18.6kDa according to NP_001271.1 and 18.2kDa according to NP_001287744.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.

Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.

Application Type
Pep-ELISA, WB, IF, FC

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