Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names MYO5A|MYR12|myoxin|myosin, heavy polypeptide kinase|myosin VA (heavy chain 12, myoxin)|myosin VA|myosin V|MYO5|MYH12|GS1|dilute | |
Usage Summary Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2031-6; PMID: 20133847). Recommended concentration: 1-3µg/ml. | |
Accession ID NP_000250.3; NP_001135967.1 | |
Blocking Peptide EBP10262 | |
Immunogen Peptide with sequence C-ETKQLELDLN, from the internal region of the protein sequence according to NP_000250.3; NP_001135967.1. | |
Product Comments This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1). | |
Peptide Sequence C-ETKQLELDLN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 4644 | |
Mouse Gene ID 17918 | |
Rat Gene ID 25017 | |
Product Grade ![]() | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3µg/ml. | |
Application Type Pep-ELISA, WB, IF |
Goat Anti-MYO5A Antibody
$423.00
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