Goat Anti-MYO5A Antibody

$423.00

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SKU: EB10262
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MYO5A|MYR12|myoxin|myosin, heavy polypeptide kinase|myosin VA (heavy chain 12, myoxin)|myosin VA|myosin V|MYO5|MYH12|GS1|dilute
Usage Summary
Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2031-6; PMID: 20133847). Recommended concentration: 1-3µg/ml.
Accession ID
NP_000250.3; NP_001135967.1
Blocking Peptide
EBP10262
Immunogen
Peptide with sequence C-ETKQLELDLN, from the internal region of the protein sequence according to NP_000250.3; NP_001135967.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).
Peptide Sequence
C-ETKQLELDLN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Reactive Species
Human, Mouse
Human Gene ID
4644
Mouse Gene ID
17918
Rat Gene ID
25017
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3µg/ml.
Application Type
Pep-ELISA, WB, IF