Goat Anti-Mucin 19 / Apomucin Antibody

$464.00

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SKU: EB10619
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
mucin apoprotein| sublingual apomucin| mucin-19| gel-forming secreted mucin-19| MUC-19| mucin 19|Muc19
Usage Summary
ICC/ IF: Positive staining in the sublingual salivary gland of the mouse, while cells remain negative in the submandibular salivary gland. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Albany, NY.
Accession ID
NP_997126.2
Blocking Peptide
EBP10619
Immunogen
Peptide with sequence ECKRSVKYNYETFQ, from the C Terminus of the protein sequence according to NP_997126.2.
Peptide Sequence
ECKRSVKYNYETFQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat
Reactive Species
Mouse
Mouse Gene ID
239611
Rat Gene ID
497227
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
A customer observed high MWt bands in salivary gland tissue from female or male C57BL/6 mice: Proteins were separated by electrophoresis through 3-8% gradient gels, transferred to nitrocellulose, and probed with primary antibody at 1:2000 dilution (0.25 µg/ml), followed by peroxidase-conjugated anti-goat at 1:50,000.
Application Type
Pep-ELISA, WB, ICC/ IF