Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names septin 4|SEP4|PNUTL2|peanut-like 2|MART|hucep-7|hCDCREL-2|H5|cerebral protein 7|cell division control-related protein 2|CE5B3|BRADEION|ARTS | |
Usage Summary Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_004565.1; NP_536340.1; NP_536341.1; NP_001185642.1 | |
Blocking Peptide EBP10428 | |
Immunogen Peptide with sequence C-ELSKFVKDFSGN, from the internal region of the protein sequence according to NP_004565.1; NP_536340.1; NP_536341.1; NP_001185642.1. | |
Product Comments This antibody is expected to recognize all reported isoforms (NP_004565.1; NP_536340.1; NP_536341.1, NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence. | |
Peptide Sequence C-ELSKFVKDFSGN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse | |
Reactive Species Human, Mouse | |
Human Gene ID 5414 | |
Mouse Gene ID 18952 | |
Product Grade ![]() | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx 55kDa band observed in nuclear lysates of cell lines Jurkat and in LNCaP cell lysates, 50-55kDa in Human Cerebellum lysates, and approx. 50kDa in Mouse Brain lysates (calculated MW of 53.0kDa according to Human NP_536341.1 and 49.4kDa according to Mouse NP_001271323.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, FC |
Goat Anti-SEPT4 (aa33-44) Antibody
$423.00
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